Section 4.2 Running An Individual Plate - Applied Biosystems 7900HT User Manual

Section 4.2 Running an Individual Plate

In This Section
Pre-Run
Checklist
Workflow
Overview
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
Saving the Plate Document . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-24
Running a Single Plate (Using the SDS Software) . . . . . . . . . . . . . . . . . . . . . . . . 4-25
After the Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-29
The following tasks must be complete to run plates on the 7900HT instrument. See
the associated page number for details on each procedure.
Done Check
A background run has been performed in the last week
A pure dye run has been performed in the last 6 months
The instrument tray does not contain a plate
IMPORTANT!
the instrument tray contains a plate, you must eject and remove it.
1. Save the SDS Plate Document (see
2. Load and run the prepared optical plate or Low Density Array (see
3. Analyze the run data.
– Allelic Discrimination Analysis (see
– Absolute Quantification Analysis (see
– Relative Quantification Analysis (see
– Dissociation Curve Analysis (see
The instrument tray must be empty to begin a run. If
page 4-24).
page 5-5).
page
page
page 6-37).
September 1, 2004 11:39 am, CH_Basic.fm

Section 4.2 Running an Individual Plate

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6-5).
6-15).
DRAFT
See Page
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4-25).
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