Analyzing The Background Calibration Data - Applied Biosystems 7300 Installation And Maintenance Manual

Chapter 5 Performing the Background Calibration and Optical Calibration

Analyzing the Background Calibration Data

Analyzing the Background Calibration Data
When the run is complete, click
1.
Click (or select Analysis Extract
2.
Background).
The software extracts the background signal,
then displays one of the following messages:
If the software displays:
Background Extraction Complete – The analysis is
successful.
Image exposure is too low... – The software stopped
the extraction because several raw spectra are at or
below the detectable threshold for the calibration.
Image exposure is too high... – The run is
unsuccessful. The software stopped the extraction
because one or more raw spectra exceed the maximum
limit for the 7300/7500/7500 Fast system.
Notes
56
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
.
Do this:
Click
Click
a. Click
b. Verify that the instrument contains the background
plate.
c. Test the lamp. See
page
d. Run the background plate again.
e. If the SDS software continues to display the Image
exposure is too low... dialog box, click
then go to
Click
"Troubleshooting" on page
Click
Select
, then go to step 4.
.
"Monitoring Lamp Status" on
117.
step 4.
, then troubleshoot the failed run. See
64.
,