Applied Biosystems 7500 Manuals

Manuals and User Guides for Applied Biosystems 7500. We have 4 Applied Biosystems 7500 manuals available for free PDF download: Installation And Maintenance Manual, Getting Started, Getting Started Manual

Applied Biosystems 7500 Installation And Maintenance Manual (174 pages)

Real-Time PCR System

Brand: Applied Biosystems | Category: Laboratory Equipment | Size: 15 MB

Applied Biosystems 7500 Getting Started (152 pages)

Real-Time PCR System

Brand: Applied Biosystems | Category: Laboratory Equipment | Size: 9 MB

Table of Contents
5
How to Use this Guide
9
Preface
9
How to Obtain more Information
11
How to Obtain Support
13
Safety Conventions Used in this Document
14
Symbols on Instruments
15
Safety Labels on Instruments
16
General Instrument Safety
18
Chemical Safety
19
Chemical Waste Safety
21
Electrical Safety
22
Physical Hazard Safety
22
Biological Hazard Safety
23
Workstation Safety
23
Safety and Electromagnetic Compatibility (EMC) Standards
24
Chapter 1 Get Started

25
Chapter 2 Design the Standard Curve Experiment

42
- Chapter Overview
  42
- Create a New Experiment
  43
- Define the Experiment Properties
  44
- Define the Methods and Materials
  46
- Set up the Targets
  48
- Set up the Standards
  50
- Set up the Samples
  52
- Set up the Run Method
  54
- Review the Reaction Setup
  56
- Order Materials for the Experiment
  61
- Finish the Design Wizard
  64
Chapter 3 Prepare the Reactions

67
- Chapter Overview
  68
- Prepare the Sample Dilutions
  69
- Prepare the Standard Dilution Series
  71
- Prepare the Reaction MIX
  73
- Prepare the Reaction Plate
  75
Chapter 4 Run the Experiment

79
- Chapter Overview
  80
- Prepare for the Run
  81
- Enable the Notification Settings (Optional)
  83
- Monitor the Run
  85
- Start the Run
  85
- Unload the Instrument
  88
Chapter 5 Analyze the Experiment

89
- Chapter Overview
  90
- Section 5.1: Review Results
  91
Section 5.1 Review Results

91
- Analyze the Experiment
  92
- View the Standard Curve
  95
- View the Amplification Plot
  98
- View the Well Table
  105
- Publish the Data
  107
- Section 5.2: Troubleshoot (if Needed)
  109
Section 5.2 Troubleshoot (if Needed)

109
- View the Analysis Settings
  110
- View the QC Summary
  112
- Omit Wells from the Analysis
  114
- View the Multicomponent Plot
  116
- View the Raw Data Plot
  118
Appendix A Alternate Experiment Workflows

121
- Advanced Setup Workflow
  122
- Quickstart Workflow
  124
- Template Workflow
  126
- Export/Import Workflow
  128
  - Bibliography
    131
  - Glossary
    133
  - Index
    147

Applied Biosystems 7500 Getting Started Manual (106 pages)

Fast Real-Time PCR System

Brand: Applied Biosystems | Category: Laboratory Equipment | Size: 4 MB

Applied Biosystems 7500 Getting Started Manual (78 pages)

Fast Real-Time PCR System

Brand: Applied Biosystems | Category: Laboratory Equipment | Size: 5 MB

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Laboratory Equipment Measuring Instruments Control Unit Controller DC Drives

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Applied Biosystems 7500 Manuals

Applied Biosystems 7500 Installation And Maintenance Manual (174 pages)

Table of Contents

Chapter 1 Before Starting the Installation

Chapter 2 Unpacking and Setting up the Instrument

Chapter 3 Installing the Software and Powering on the Instrument

Chapter 4 Performing the Instrument Function Test

Chapter 6 Performing the Pure Dye Calibration

Chapter 7 Verifying the Instrument Performance

Chapter 5 Performing the Verification Run

Appendix A Maintaining the Instrument

Appendix B Creating a Background Plate

Appendix C Creating a Custom Pure Dye Plate

Appendix D Setting up the Computer

Applied Biosystems 7500 Getting Started (152 pages)

Table of Contents

Chapter 1 Get Started

Chapter 2 Design the Standard Curve Experiment

Chapter 3 Prepare the Reactions

Chapter 4 Run the Experiment

Chapter 5 Analyze the Experiment

Section 5.1 Review Results

Section 5.2 Troubleshoot (if Needed)

Appendix A Alternate Experiment Workflows

Applied Biosystems 7500 Getting Started Manual (106 pages)

Table of Contents

Chapter 1 Introduction

Chapter 2 Designing an RQ Experiment

Chapter 3 Performing Reverse Transcription

Chapter 4 Generating Data from RQ Plates - 7300 or Standard 7500 System

Chapter 5 Generating Data from RQ Plates - 7500 Fast System

Chapter 6 Analyzing Data in an RQ Study

Appendix A Creating Detectors

Appendix B Example RQ Experiment

Applied Biosystems 7500 Getting Started Manual (78 pages)

Table of Contents

Chapter 1 Introduction

Chapter 2 Designing a Plus/Minus Experiment

Chapter 3 Setting up the Reaction Plate

Chapter 4 Performing the Plus/Minus Pre-Read Run

Chapter 5 Generating Amplification Data

Chapter 6 Performing the Plus/Minus Post-Read Run

Appendix A Creating Detectors

Appendix B Viewing Amplification Data

Appendix C Example Plus/Minus Experiment

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