Applied Biosystems 7900HT User Manual page 382

single-plex TaqMan reagents
TaqMan primers and probes
trimming after sealing
TaqMan reagents, description
TaqMan RNase P Instrument Verification Plates
about 7-30
analyzing 7-34
kits C-5
preparing a plate document
running 7-30, 7-32
Targets, mRNA, number per well
tasks, See detector tasks
templates
about 2-12
creating a single plate document
creating multiple plate documents
saving as 3-24
thermal cycler block, See sample block module
thermal cycling protocol, See methods
threshold
about D-6
configuring for automatic analysis
improper setting 8-5
threshold cycle
calculation D-6
relationship to PCR product
Time readout (from the Real Time tab)
Time Remaining readout (from the Real Time
tab) 4-27
T , See melting temperature
m
troubleshooting 8-2 to
7900HT instrument
background runs 8-9
chemistry problems
computer 8-14
end-point runs 8-13
fixed-position bar code reader 8-17,
pure dye runs 8-11
real-time runs 8-12
SDS software 8-14
Zymark Twister Microplate Handler 8-17,
turning ON the Applied Biosystems 7900HT Fast Real-
Time PCR System
U
upgrading
operating system software
SDS software 7-55
V
viewing, well information
views
hiding 2-21
maximizing/minimizing
Index-8 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
September 1, 2004 11:38 am, 4351684AIX.fm
4-11
4-10
4-21
4-10
7-33
4-10
3-26
4-36
6-11
D-7
4-27
8-20
8-14
8-5 to 8-8
8-20
8-20
2-4
7-55
2-23
2-22
DRAFT
resizing 2-21, 2-22
showing 2-21
W
warranty statement E-1
well inspector 2-31
Y
Y Inter readout (from the Standard Curve Plot)
Z
zooming
allelic discrimination plot
plate grid wells 2-26
Zymark Twister Microplate Handler
See Plate Handler
Zymark Twister Software
about 1-14
aligning the Plate Handler
closing 7-48
launching
6-13
5-14
7-41 to 7-48