Analyzing The Data - Applied Biosystems 7900HT User Manual

Analyzing the Data

User Access
Requirement
Configuring the
Analysis Settings
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
There is no access requirement. All users can analyze absolute quantification data
that has been saved to the SDS Enterprise Database.
Before analyzing the plate document data, you must configure the analysis settings
for each detector that you want to analyze. The analysis settings are detector-specific
and must be set for each one individually. For each detector, the SDS software can
calculate baseline and threshold values automatically using the AutoC
manually using values you supply.
You can analyze the run without configuring the analysis settings, however the
Note:
software will analyze the data using a default baseline of cycle 3 to 15 and a default
threshold value of 0.2.
1. Click (or select Analysis menu > Analysis Settings).
2. In the Detector drop-down list, select a detector.
3. In the Analysis Settings dialog box, configure the method (automatic or manual)
that the software will use to determine the baseline and threshold values for the
selected detectors:
To determine the baseline and threshold values:
Automatically – Select the Automatic C
If you choose to use the AutoC
IMPORTANT!
baseline and threshold were called correctly for each autocalled detector
following the analysis (use the procedure on
See "Automatic Threshold and Baseline Determination" on page 6-18
Note:
more information on the AutoC
Manually – Do the following:
a. Select the Manual Ct option button.
b. In the Threshold field, enter a threshold value to apply to the selected
detector(s) or leave the field empty and set the threshold value manually as
explained on
T
algorithm.
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page 6-46.
September 1, 2004 11:39 am, CH_Real-Time.fm
Analyzing the Data
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option button.
algorithm, verify that the
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page 6-46).
DRAFT
algorithm, or
for
6-11