Applied Biosystems 7900HT User Manual page 368

SDS 7900HT
Document (*.sds)
SDS 7900HT
Template
Document (*.sdt)
absolute
quantification (AQ)
allele
allelic
discrimination (AD)
amplicon
Amplification plot
analysis session
annealing
Application
Program Interface
(API)
assay type
auto increment
value
Glossary-2 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
September 1, 2004 11:39 am, Glossary.fm
A file used by the SDS software to store plate documentation locally on a computer
hard drive. A plate document is a virtual representation of a consumable (plate) used
to contain samples and reagents during a sequence detection run. During the run, the
software uses the plate document to record the operation of the instrument (thermal
cycling and data collection parameters), organize and store the data gathered during
the PCR process, store the of the run data, and record analysis and display options.
Plate document templates have the same format as the SDS 7900HT Documents
(*.sds) but do not store raw data or bar codes.
The objective of an absolute quantification experiment is to accurately determine the
absolute quantity of a single nucleic acid target sequence within an unknown sample.
The results of an absolute quantification experiment are reported in the same units of
measure as the standard used to make them.
One of the variant forms of a gene at a particular locus or location on a chromosome
The process by which two variants of a single nucleic acid sequence present in a
population of individuals' DNA samples is determined. Single nucleotide
polymorphisms (SNPs) comprise the most common variants that are assayed using
TaqMan reagents.
A PCR amplified section of DNA
The Amplification plot displays data from real-time PCR for gene expression assays
after signal normalization and multi-component analysis. The Amplification plot
appears only in plate documents containing analyzed data from real-time runs. It is
a 2D plot of normalized reporter fluorescence (for ∆R
number. The ∆R
versus cycle Amplification plot is used for calculation threshold
n
cycle (C ).
T
A set of analysis results derived from a single raw data set associated with a single
plate document analyzed by the SDS software. The analysis session is persisted in the
database and can be retrieved by the SDS software. Multiple analysis sessions can be
created from the same plate document, however each analysis session must have a
unique name. An analysis session does not have to be associated with a Study;
however, if it has such a relationship, it can be associated with at most one Study at
any given time.
Binding a probe to the DNA or cDNA during the PCR process
A set of routines, protocols, and tools for building software applications. A good API
makes it easier for a programmer to develop a program by providing all the building
blocks necessary to link the elements of the various subcomponents of the system.
With reference to the SDS 2.2 Enterprise Option software, an assay type is one of the
following: allelic discrimination, absolute quantification, or relative quantification.
Method setting that increases or decreases the value for any PCR set-point parameter
(time or temperature) by a fixed amount every cycle.
DRAFT
) values versus the cycle
n