After The Run - Applied Biosystems 7900HT User Manual

After the Run

User Access
Requirement
Ejecting a Plate
(Opening and
Closing the
Instrument Tray)
Saving the
Run Data
Analyzing the
Run Data
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
If using the SDS Enterprise Database, you must belong to the Scientist or
Administrator User Group to save a plate document.
View the instrument status icon to determine the plate document to use to eject the plate.
Instrument Status Action
1. In the SDS software, select Window > (the plate document
Connected to 2. In the plate document, select the Instrument tab
platename
3. Select the Plate-Read or Real-Time tab.
4. Click
1. In the SDS software, click
2. Click
Disconnected
3. Select the Instrument tab.
4. Select the Plate-Read or Real-Time tab.
5. Click
After the run, you must either save the results to the SDS Enterprise Database or save
the run data to the SDS 7900HT Document (*.sds).
To save the run data to the SDS 7900HT Document:
1. In the SDS software, select File > Save.
To save the run data to the SDS Enterprise Database:
If you saved the plate document to the database prior to running the
IMPORTANT!
plate, the SDS software stored the run data as a temporary file in the /temp directory. If
you closed your plate document at the end of the run without saving it, open the temp
directory and rename the temporary file to *.sds and you will be able to open the file.
1. Select File > Save Document to Database:
2. In the Comment field of the Save Document to Database dialog box, enter a
brief description of the plate document (up to 255-characters).
If saving a plate document file to the database, you will loose the file
Note:
name unless you enter it into the Comments field.
3. Click .
4. In the Saved Document dialog box, click
Section 5.1 Allelic Discrimination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5
Section 6.1 Absolute Quantification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-5
Section 6.2 Relative Quantification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-15
Section 6.3 Dissociation Curve Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-37
connected to the instrument).
.
.
.
DRAFT
September 1, 2004 11:39 am, CH_Basic.fm

After the Run

(or select File > New).
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