Applied Biosystems 7900HT User Manual page 374

template
threshold
threshold cycle (C )
T
T
m
Glossary-8 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
September 1, 2004 11:39 am, Glossary.fm
(1) [SDS software: SDS 7900HT Template Document or plate document template] A
pattern for creating an SDS 7900HT Document (plate document). The filename
extension for a plate document template is *.sdt. The plate document template
facilitates creation of plate documents in the SDS software. It is typically a starting
point for batch document setup. The binary representation of the plate document
template is persisted in the database, allowing the plate document template to be
retrieved and then populated with data and saved as an SDS 7900HT Document.
(2) [Chemical] the DNA sample that is being amplified during PCR.
An arbitrary level of normalized reporter signal (R
assays. The level is set to be above the baseline and sufficiently low to be within the
exponential growth region of the amplification region of an amplification curve. The
threshold is the line whose intersection with the Amplification plot defines the C
For a given well, the threshold cycle (C
software first detects a noticeable increase in reporter fluorescence above a baseline
signal.
(1)[Chemical] The temperature at which 50% of the DNA amplicons are in a
double-stranded configuration. (2) [Mathematical] The maximum value for the first
derivative curve of normalized reporter fluorescence (R
DRAFT
) for C determination in real-time
n T
) represents the PCR cycle at which the SDS
T
).
n
.
T