About The Centrifuge System - Applied Biosystems 7900HT User Manual

Chapter 4 Operating the Instrument

About the Centrifuge System

Required
Centrifuge and
Accessories
Centrifuge
System
Components
4-12 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
September 1, 2004 11:39 am, CH_Basic.fm
After the fill reservoirs of a Low Density Array have been loaded with the cDNA
sample or control, the centrifuge system is required to distribute the cDNA sample or
control to the reaction wells. The centrifuge system supports the simultaneous
loading of up to 12 Low Density Arrays.
To properly centrifuge the Low Density Arrays, you will need the following
centrifuge and centrifuge accessories:
• Sorvall ® Legend T Centrifuge
GR2170
Figure 4-7 Sorvall Legend T Centrifuge
• Sorvall ® /Heraeus "Tool-less" 750-mL Swing-Out Rotor Body
• A set of Sorvall ®
(Contact your Applied Biosystems representative to order the custom buckets.)
• Current firmware
If you are upgrading an existing Sorvall Legend T or Legend RT Centrifuge,
you may also need to upgrade the firmware so that it can recognize the Low
Density Array bucket type.
Figure 4-8 illustrates the centrifuge system components.
Figure 4-8 Centrifuge System Components
DRAFT
/Heraeus Custom Buckets (four), each with its own card holder
Centrifuge rotor
Provides the rotational drive for
a set of four centrifuge buckets
Card holder
Supports the Low Density
Array's fill reservoirs during
centrifugation
Bucket
Contains the Low Density
Arrays and card holder
during centrifugation