Applied Biosystems GeneAmp PCR System 9600 Manuals

Manuals and User Guides for Applied Biosystems GeneAmp PCR System 9600. We have 2 Applied Biosystems GeneAmp PCR System 9600 manuals available for free PDF download: User Manual

Applied Biosystems GeneAmp PCR System 9600 User Manual

Applied Biosystems GeneAmp PCR System 9600 User Manual (296 pages)

Brand: Applied Biosystems | Category: Laboratory Equipment | Size: 12 MB

Table of Contents

36
- Chapter 6. Running PCR Samples
  
  24
  - Table of Contents
    36
Table of Contents

45
- Preface: Using the Manual
  48
  - Using the Manual
    48
  - Conventions
    50
  - Software Conventions
    50
  - Notes and Warnings
    51
- Chapter 1. Introducing the Geneamp ® PCR System 9600
  
  52
  - Introduction
    52
  - Principles of Operation
    53
  - Instrument Description
    54
    
    A Look at the Sample Block
    54
    
    A Look at the Control Panel
    55
    
    Instrument Features
    56
    
    The Geneamp PCR System 9600 Software
    56
- Chapter 2. Installing the Geneamp PCR System 9600
  
  58
  - Introduction
    58
  - Unpacking
    59
  - Parts Supplied
    59
  - Supplies, Accessories, and Replacement Parts
    60
  - Power Requirements
    61
  - Proper Location
    62
    
    Temperature, Humidity, and Environment
    62
  - Setting up the Printer
    63
    
    Installing the 115-VAC Printer
    63
    
    Installing the 230-VAC Printer
    67
    
    Configuring the Printer
    69
  - Configuring the Geneamp PCR System 9600
    71
    
    Summary of Configuration Parameters
    72
  - Heater and Chiller Tests
    76
    
    Heater Test
    76
    
    Chiller Test
    78
- Chapter 3 Tutorial: Customizing a PCR CYCL Program
  
  80
  - Introduction
    80
  - Before You Start
    81
  - What Is a CYCL Program
    82
  - Starting up the Instrument
    84
  - Getting to the CYCL Program
    85
  - A Look at the CYCL Program
    88
  - Customizing the CYCL Program
    93
    
    Defining the Number of Setpoints
    93
    
    Defining Values for Setpoint # 1
    94
    
    Defining Values for Setpoint #2
    96
    
    Defining Values for Setpoint #3
    96
    
    Specifying the Number of Cycles
    97
  - Storing Your CYCL Program
    99
  - Starting a Trial Run
    101
    
    Loading a Tray (Without Samples)
    101
    
    Running the Program
    103
  - The Next Step
    106
  - Software Basics
    108
    
    Introduction
    108
- Chapter 4. Software Basics
  
  108
  - Reviewing Keypad Basics
    109
    
    Use of the Keys
    110
  - A Close Look at the Main Menu
    111
    
    Selecting a Function
    112
  - An Overview of PCR Programs and Methods
    113
    
    Linking Programs in a PCR Method
    113
    
    Storing Programs and Methods
    114
  - The HOLD Program
    115
  - CYCL: the Cycle Program
    117
  - The AUTO Program
    119
  - PCR Methods
    121
- Chapter 5 Setting up PCR Programs and Methods
  
  122
  - Introduction
    122
  - Setting Hold Times on the Geneamp ® PCR System 9600
    123
  - Cycler to Geneamp PCR System 9600)
    126
    
    Editing Keys
    129
    
    Creating and Editing Programs
    130
    
    Creating a Program
    131
    
    Storing the Program
    132
    
    Editing a Program
    134
    
    Printing a Program
    137
    
    Creating and Editing Methods
    138
    
    Creating a Method
    139
    
    Storing the Method
    142
    
    Editing a Method
    144
    
    Printing a Method
    146
  - Sample Conversion of Hold Times for up Ramps (DNA Thermal Cycler to Geneamp PCR System 9600)
    126
  - Example of a Method Printout Using PROGRAM DATA
    148
    
    Software Shortcuts
    149
    
    Using the STOP Key
    149
    
    Recalling Programs: a Shortcut
    151
    
    The Displays in a HOLD Program
    152
    
    The HOLD Program
    152
    
    Description of HOLD Program Displays
    153
    
    CYCL: the Cycle Program
    154
    
    The Displays in a CYCL Program
    154
    
    The Main Displays in a Three-Temperature CYCL Program
    154
    
    Description of CYCL Program Displays
    155
    
    The AUTO Program
    160
    
    The Displays in an AUTO Program
    160
    
    Description of AUTO Program Displays
    161
  - Running PCR Samples
    168
    
    Introduction
    168
    
    Loading Samples
    169
    
    Using the 96-Position Sample Tray with Microamp Reaction Tubes
    169
  - Setting up the 96-Position Sample Tray
    170
  - Securing the Retainer on the Tray
    171
    
    Placing the Sample Tray in the Block
    172
  - Sliding the Heated Cover Forward
    173
  - Turning the Cover Knob
    174
    
    Removing the Sample Tray from the Block
    175
    
    Using the Cap-Installing Tool
    175
    
    Removing Samples from the Tray
    176
    
    Create a New Program or Method
    177
    
    Edit an Existing Program or Method
    177
    
    Recall a Stored Program or Method
    177
    
    Specifying a Program or Method
    177
    
    Run Keys
    178
    
    Run-Time Keys
    178
    
    Starting the Run
    178
    
    Running a Stored Program
    179
  - An Example Printout of a CYCL Program Using the SETPOINT Option
    182
    
    Indicator Lights
    183
    
    CYCL and AUTO Program Run-Time Displays
    184
    
    Run-Time Displays
    184
    
    HOLD Program Run-Time Display
    186
    
    Pausing a Run
    187
    
    Stopping a Run
    187
    
    Reviewing the History of the Run
    189
  - Utilities
    190
    
    Introduction
    190
- Chapter 7. Utilities
  
  190
  - Getting to the Utilities Menu
    191
  - Selecting a Utilities Function
    192
  - The Utilities Functions
    192
  - The Directory Function (DIR)
    193
    
    Listing Programs by Their Program Number (PROG)
    193
    
    Listing Programs by Their Program Type (TYPE)
    196
  - Example of a List of Programs Listed According to Program Type
    198
    
    Listing Programs by Their User Number (USER)
    199
  - Example of a List of Programs Listed According to User Number
    201
    
    Printing a Directory of Your Programs (PRINT)
    202
    
    Editing the Configuration Parameters
    205
    
    The Configuration Function
    205
    
    The Configuration Function (CONFIG)
    205
    
    Setting the Time and Date
    207
    
    Turning the Run-Time Printer Mode and Run-Time Beeper on or off
    209
    
    Changing the Pause Time-Out Limit
    211
    
    Changing the Allowed Setpoint Error
    212
    
    Entering an Idle State Setpoint Value
    214
    
    Changing the Temperature Interval for the Setpoint Clock
    215
    
    Changing the Sample Tube Type
    217
    
    MICRO Time Constant
    219
    
    Setting Sample Tube Time Constants
    219
    
    THIN Time Constant
    221
    
    Printing the Configuration Parameters
    223
    
    The Diagnostic Function (DIAG)
    224
    
    Selecting the Diagnostic Tests
    225
    
    About the History File
    227
    
    Reviewing the History File
    227
    
    Status Records in a History File
    228
  - A Typical Status Record Display
    230
    
    Data Records in a History File
    231
    
    Displaying the History File
    233
    
    Messages (ERROR), and Printing Records (PRINT)
    236
    
    History File
    237
    
    Printing the History File
    237
    
    Running the Heater Test
    239
    
    Running the Chiller Test
    241
    
    Running the System Performance Test
    243
    
    Running the Verify Calibration Test
    245
    
    Deleting Programs by Program Number
    246
    
    The Delete Function (DEL)
    246
    
    Deleting Programs by User Number
    248
    
    Deleting All Unprotected Programs
    250
  - Maintenance
    252
    
    Introduction
    252
- Chapter 8. Maintenance
  
  252
  - Cleaning the Sample Wells
    253
  - Cleaning the Heated Cover
    254
  - Temperature Calibration Verification Test
    255
    
    Equipment Required
    255
    
    Verifying the Temperature Calibration
    255
  - Location of Sample Wells E1 and D1
    256
  - Temperature Measurements in the Temperature Calibration
    260
    
    Verification Test
    260
    
    Geneamp PCR System 9600 Calibration Label
    261
    
    Test Results
    261
    
    Equipment Required
    264
    
    Temperature Uniformity Test
    264
    
    Testing the Temperature Uniformity
    264
  - Location of Wells Used in Uniformity Test
    267
    
    Test Results
    268
    
    Changing External Fuses
    269
    
    Removing the Fuses
    269
- Chapter 9. Troubleshooting
  
  270
  - Introduction
    270
  - Troubleshooting
    270
  - If the Instrument Turns off
    274
- Chapter 10. Specifications
  
  276
  - Dimensions
    276
  - Sample Compartment
    276
    
    Sample Tubes
    276
  - Power Requirements
    277
  - Control Panel
    277
    
    Display
    277
    
    Indicator Lights
    277
    
    Keypad
    277
  - Sample Temperature Range
    278
  - Sample Temperature Accuracy
    278
  - Displayed Sample Temperature
    278
  - Sample Block Temperature Uniformity
    278
  - Sample Temperature Overshoot
    278
  - Ramp Time Repeatability
    279
  - Sample Heating and Cooling Rate
    279
  - Printer Interface
    280
  - Temperature Calibration
    280
  - Index
    282

Applied Biosystems GeneAmp PCR System 9600 User Manual (151 pages)

Thermal Cycler Temperature Verification System

Brand: Applied Biosystems | Category: Laboratory Equipment | Size: 3 MB

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Applied Biosystems GeneAmp PCR System 9600 Manuals

Applied Biosystems GeneAmp PCR System 9600 User Manual (296 pages)

Table of Contents

Table of Contents

Chapter 6. Running PCR Samples

Table of Contents

Chapter 1. Introducing the Geneamp ® PCR System 9600

Chapter 2. Installing the Geneamp PCR System 9600

Chapter 3 Tutorial: Customizing a PCR CYCL Program

Chapter 4. Software Basics

Chapter 5 Setting up PCR Programs and Methods

Chapter 7. Utilities

Chapter 8. Maintenance

Chapter 9. Troubleshooting

Chapter 10. Specifications

Applied Biosystems GeneAmp PCR System 9600 User Manual (151 pages)

Table of Contents

Table of Contents

Introduction 1

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® Temperature Verification Tests for the Geneamp 9700, 0.5-Ml Sample Block Module 7

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Temperature Verification Tests for the Geneamp 9600 9

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