Analysis Checklist - Applied Biosystems 7900HT User Manual

Analysis Checklist

Workflow
Overview
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
1. Open the plate document (see
2. Configure the analysis settings for the run (see
3. Analyze the run data (see
4. For each detector that you configured to determine the baseline and threshold
values (see page 6-46):
– Automatically – Verify that the baseline and threshold values for the detector
were set correctly by the software.
– Manually – Set the baseline and threshold values for the detector
5. Visualize outliers and eliminate any outlying amplification from the run data
(see page 6-49).
6. View the results of the absolute quantification run (see
7. Choose from the following post-analysis options (see
– Reanalyze the run data.
– Adjust the display settings for the results table, plate grid, and plate
document plots.
– Print elements of the plate document.
– Export the plate document results table or plots.
– Save the analysis.
page 6-10).
page
page 6-12).
DRAFT
September 1, 2004 11:39 am, CH_Real-Time.fm

Analysis Checklist

6-11).
page 6-14).
page 6-52):
6-9