Applied Biosystems 7900HT User Manual page 258

Chapter 7 Maintaining the Instrument
7-26 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
September 1, 2004 11:39 am, CH_Maintenance.fm
b. If the data set contains an outlying peak, eliminate it by clicking the check
box of the associated well.
Dye spectra are generally acceptable if they peak at the same location as
Note:
their group but diverge slightly at other wavelengths.
c. Click
d. Repeat steps a to c for all remaining wells until prompted with a message
reporting the extraction of the pure dyes.
The software extracts the pure spectra and stores the data as a component of the
calibration file.
3. Click (or select File > Save).
The software saves the plate document.
4. Select File > Close.
The software closes the plate document.
5. If performing spectral calibration of a:
• Standard 384-Well Block – The pure dye calibration is complete.
• Standard 96-Well Block – Run the second Pure Dye plate by repeating the
procedures for:
– "Preparing a Plate Document for a Pure Dye Plate or Card" on page 7-22
– "Running the Prepared Pure Dye Plate or Card" on page 7-24
– "Analyzing the Pure Dye Run" on page 7-25
• 7900HT System TaqMan Low Density Array Upgrade – Run the second
and third microfluidic pure dye cards containing the VIC and ROX dyes by
repeating the procedures for:
– "Preparing a Plate Document for a Pure Dye Plate or Card" on page 7-22
– "Running the Prepared Pure Dye Plate or Card" on page 7-24
– "Analyzing the Pure Dye Run" on page 7-25
DRAFT
when finished.