Applied Biosystems 7900HT User Manual page 106

Chapter 3 Preparing a Run
3-22 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
September 1, 2004 11:39 am, CH_Run.fm
4. Configure the data collection options for the method:
a. Select the Data Collection tab.
b. Click below each plateau or ramp inside the cycle stage of the thermal
profile to place a data collection icon at each step.
Configure the method of your plate document to collect data only
IMPORTANT!
in the necessary stages of the PCR. The file size for a real-time run depends on
the length of the run and the configuration and placement of the data collection
icons. Longer runs and runs configured to collect data at multiple stages of the
method can be considerably larger than the 15–25 MB average.
5. Click the Sample Volume (µL) field and enter the volume of the reactions on
the plate.
Sample volume refers to the entire contents of any well, including buffer
Note:
blank, or any combination of master mix and nucleic acids.
All wells on one plate must contain the same reaction volume.
IMPORTANT!
6. Choose from the following options:
If performing an...
absolute or relative
quantification run only
absolute quantification run
with a dissociation curve
DRAFT
Data collection icons
Then...
go to "Step 6 – Saving the Plate Document as a
Template" on page 3-24.
add a temperature ramp to generate dissociation
curve data as explained on page 3-23.