Generating Data From Rq Plates - 7300 Or Standard 7500 System - Applied Biosystems 7500 Getting Started Manual

STANDARD STANDARD STANDARD
Primer Extended on mRNA
5´
Oligo d(T) or random hexamer
Synthesis of 1st cDNA strand
3´
Generating
Data from
STANDARD
RQ Plates
Standard
Notes
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Relative Quantification Getting Started Guide
Generating Data from RQ Plates –
7300 or Standard 7500 System
Introduction
and Example
RQ Experiment
Designing
an RQ
Experiment
Performing
3´ Reverse
5´ cDNA
Reverse
Primer
5´ cDNA
Transcription
Generating
Data from
OR
RQ Plates
Fast
Performing
an RQ Study
Chapter 4
Prepare the
PCR Master Mix
Prepare the
reaction plate
Create a new
RQ Plate document
Program the
thermal cycling conditions
Save the
RQ Plate document
Start the run
View RQ plate data
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4
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