Applied Biosystems 7900HT User Manual page 91

Allelic
Discrimination
Workflow
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
1. Create an allelic discrimination plate document (see
2. * Apply markers to the plate document:
a. Create detectors for the allelic discrimination probes (see
b. Create a marker for each allelic discrimination probe pairing (see
c. Copy the marker(s) to the plate document (see
3. * Assign detector tasks to the wells of the plate document (NTC and Unknown)
(see page 3-16).
4. If you would like to perform thermal cycling of the allelic discrimination plate on
the 7900HT instrument, create a real-time plate document for the plate and
program it with the method for the allelic discrimination run. Otherwise, continue
to step 2 (see page 3-19).
5. Choose from the following:
– If running a single plate, then continue to
– If running the first plate in a series of plates with identical assay
configurations, then save the plate document as a template (see
6. Create a plate document from the template created in
7. Configure the document with sample names and plate information
(see page 3-28).
a. Prepare the allelic discrimination plate or plates and perform thermal cycling
on a designated thermal cycler (see
b. Run the allelic discrimination plate or plates on the 7900HT instrument.
8. Analyze the run data (see
*Steps 2 and 2 can be eliminated by importing the plate document setup information
from a tab-delimited text file. See
on page A-2 for more information.
page
step 2.
page 4-1).
page 5-5).
"Importing Plate Document Setup Table Files"
DRAFT
September 1, 2004 11:39 am, CH_Run.fm
Workflow Overview
page 3-9).
page 3-11).
page 3-14).
3-15).
page 3-24).
step 2 (see page 3-26).
3-7