Applied Biosystems 7900HT User Manual page 377

fluorescent, common sources
isolating on the sample block module
contextual menus, using
Control assay 4-11
copying
detectors to a plate document
markers to a plate document
Cover readout (from the Real Time tab)
creating
background plate documents
custom pure dye plate documents
detectors 3-11
markers 3-14
plate documents 2-13,
plate documents from a template 3-26,
pure dye plate documents
C , See threshold cycle
T
custom pure dyes
adding to the pure dye set
creating a custom pure dye plate
cycle set, adding to a method
D
data type definitions (exportable)
database
saving plate documents
saving results 5-19,
saving templates 3-25
database connection A-22
decontaminating the sample block module
defragmenting the hard drive
deleting, steps from a method
detector tasks
about 3-16
applying 3-16
importing into a plate document
detectors
about 3-11
applying to a plate document
copying to a plate document
creating 3-11
importing into a plate document
tasks, See detector tasks
determining melting temperatures
diagram
database LAN setup
database WAN setup
disconnecting the SDS software
display settings, adjusting
dissociation curve
about 6-38
analyzing 6-40 to 6-44
definitions of the T
m
procedure checklist 6-22,
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
8-7
8-9
2-28
3-13
3-15
4-27
7-17
7-28
3-9
4-36
7-22
7-27 to 7-29
7-27
3-21
A-17
4-24
6-53
7-14
7-54
3-21
A-2
3-13
3-13
A-2
6-44
1-23
1-24
4-30
3-24
value 6-43
6-40
programming a temperature ramp
Dissociation Plot
about
exporting as a graphic file
exporting data as a text file
E
ejecting a plate from the 7900HT instrument 4-29,
endogenous control
end-point runs
about
allelic discrimination, See allelic discrimination
entering
bar code information 2-27, 3-28,
comments 3-11,
sample names into a plate document
Equipment needed
exportable data type definitions
exporting
data from a plate document 2-14,
plots and views as graphic files
External endogenous control assay
F
finger pads
cleaning
replacing
fixed-position bar code reader
to 7-15
aligning
connections
location
specification
fluorescent
contamination
detection system
fluorogenic probe
about
designing
G
gene expression level
maximum
minimum
gene expression levels
Gene Expression Plot
Genomic DNA contamination, minimizing
genotypic segregation of datapoints
(Allelic Discrimination Plot)
Geometric (Exponential) Phase
graph, Gene Expression Profile
sample bars
X-axis
September 1, 2004 11:38 am, 4351684AIX.fm
6-42
A-16
4-44
6-20
5-2
3-28
4-12
7-52
7-52
7-49 to 7-51
1-10
1-7
1-7
8-7
1-10
D-2
B-2 to B-3
6-33
6-33
6-31
6-31
6-31
6-31
DRAFT
3-23
A-16
4-36
3-28
A-17
A-16
A-16
4-11
4-11
5-8
D-4
Index-3