Applied Biosystems StepOne Manuals

Applied Biosystems StepOne Manuals

Manuals and User Guides for Applied Biosystems StepOne. We have 1 Applied Biosystems StepOne manual available for free PDF download: Installation, Networking, And Maintenance

Applied Biosystems StepOne Installation, Networking, And Maintenance

Applied Biosystems StepOne Installation, Networking, And Maintenance (184 pages)

Real-Time

Brand: Applied Biosystems | Category: Laboratory Equipment | Size: 16 MB

Table of Contents

Table of Contents
5
Preface
9
Purpose of this Guide
9
How to Obtain more Information
11
How to Obtain Support
12
Safety Conventions Used in this Document
13
Symbols on the Instruments
14
Safety Labels on Instruments
15
General Instrument Safety
16
Chemical Safety
17
Chemical Waste Safety
19
Electrical Safety
20
LED Safety
20
Biological Hazard Safety
21
Workstation Safety
21
Safety and Electromagnetic Compatibility (EMC) Standards
22
Chapter 1 Get Started

23
- Get Started
  23
- About the Stepone and Steponeplus Systems
  24
  - About the Stepone ™ and Steponeplus
    24
  - Systems
    24
- Before You Begin
  27
- Select Protective Hardware and Software
  29
- Select an Installation Layout
  31
- Plan the Installation
  33
Chapter 2 Set up the Instrument

35
- Real-Time PCR System Software
  35
- About the Installation
  36
- Unpack the Instrument
  37
- Check the Shipped Materials
  39
- Set up the Instrument
  42
- Complete the Installation
  45
Chapter 3 Install the Colocated Layout

47
- About the Colocated Installation
  48
- Install the Computer
  49
- Connect the System Components
  57
- Perform the Rnase P Experiment
  60
  - Set up the Experiment
    62
  - Run the Experiment
    63
  - Analyze the Experiment
    65
- After the Installation
  69
Chapter 4 Install the Standalone Layout

71
- About the Standalone Installation
  72
- Set up the Computer
  73
- Perform the Rnase P Experiment
  82
  - Set up the Experiment
    84
  - Run the Experiment
    85
- Analyze the Rnase P Experiment
  87
  - Unload the Instrument
    87
  - Transfer the Experiment
    88
  - Analyze the Experiment
    90
- After the Installation
  93
Chapter 5 Connect the System to a Network

95
- Help from Within the Applied Biosystems Stepone
  95
By Pressing F1, or Clicking

95
- Overview
  96
- Connect the Instrument to a Network
  100
- Set up a Computer for Remote Monitoring
  102
- Monitor the Instrument
  104
Chapter 6 Maintain the System

109
- Regular Maintenance
  110
  - Perform a Spatial Calibration
    112
  - Perform a Background Calibration
    116
  - Perform a Dye Calibration
    122
  - Archive and Back up Data
    135
- Infrequent Maintenance
  136
  - Decontaminate the Sample Block
    137
  - Decontaminate the Sample Block(S)
    137
  - Move the Instrument
    140
  - Replace the External Fuses
    142
  - Ship the Instrument for Service
    144
  - Update the Windows Operating System
    146
  - Update the Stepone Software
    147
  - Update the Stepone
    147
Appendix A How to Troubleshoot the Installation

149
- Stepone ™ Software Problems
  150
- Network Connection Problems
  151
- Background Calibration Failure
  154
- Dye Calibration Failure
  156
- Glossary
  159
- Index
  175

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