Applied Biosystems 7900HT User Manual page 96

Chapter 3 Preparing a Run
3-12 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
September 1, 2004 11:39 am, CH_Run.fm
Allelic Discrimination
Detector
4. Click .
The software saves the new detector and displays it in the detector list.
If using the SDS Enterprise Database, you cannot create a detector that
Note:
that uses the same combination of detector name, reporter dye, and quencher
dye as an existing detector.
5. Repeat steps 2 through 4
Click the
Note:
or to view the procedures for editing, deleting, or searching for detectors.
6. Choose from the following:
If constructing a
plate document for...
• absolute quantification
• relative quantification
• dissociation curve analysis
allelic discrimination
DRAFT
to create detectors for all remaining assays on the plate.
button for information on the Detector Manager dialog box
Then...
copy the detector(s) to the plate document as
explained in the procedure on
create and apply markers to the plate
document as explained on
Absolute or Relative
Quantification Detector
page 3-13.
page 3-14.