Applied Biosystems 7900HT User Manual page 228

Chapter 6 Analyzing Real-Time Data
Eliminating Wells
from the Analysis
Selected wells
(removed from use)
6-50 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
September 1, 2004 11:39 am, CH_Real-Time.fm
• If analyzing a Relative Quantification Multiple Plate Document, view the
results of the run displayed within the Gene Expression plot and
Results table.
The SDS software allows you to omit wells from the analysis. If you have identified
outliers or data you want to exclude from the analysis, follow the procedure below to
remove the corresponding wells from use.
If a well is removed from use before the plate document is run, the
IMPORTANT!
SDS software does not collect data for the well during the run. However, if a well is
removed from use after the plate document has been run, the software excludes the
well from the analysis but does not delete the data.
1. If omitting wells from a Relative Quantification Multiple Plate Document,
select a plate in the Plates field that contains wells you want to omit.
2. While pressing and holding the Ctrl key, select the well(s) in the plate grid that
you want to omit.
3. If omitting wells from a Relative Quantification Multiple Plate Document,
repeat steps 1 through 2
4. Select the Setup tab.
5. In the well inspector, click the Omit Well(s) check box to exclude the selected
wells from the analysis.
To reinstate an omitted well, select the well, then click the Omit Well(s)
Note:
check box.
DRAFT
for each plate in the study.
Omit Well(s) check box
(selected)