Running The Instrument - Applied Biosystems 7900HT User Manual

Running the Instrument

Starting the
Plate Queue
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
After you have loaded all plate documents into the Plate Queue or saved them the
SDS Enterprise Database, and you have configured the Instrument Control options,
you can start the plate queue.
To begin the plate queue, click
The Automation Controller Software does the following:
1. The Zymark Twister Microplate Handler loads the first plate from Stack 1 and
places it into plate tray for the 7900HT instrument.
2. The fixed-position bar code reader scans the bar code for the plate.
3. The Automation Controller Software does one of the following:
• If linked to the SDS Enterprise Database, the software queries the database
for the plate document with the bar code of the associated plate. When
found, the software automatically downloads the plate document.
If the software does not find a plate document with a matching bar code in
the database, the software searches the contents of the plate queue as
explained below.
• If not linked to a database, the software searches the plate queue for the
plate document with the bar code of the associated plate. If found, the
Automation Controller Software loads the plate document information
from the file system for the run.
4. The 7900HT instrument loads the instrument tray and runs the plate according
to the parameters defined by the plate document file.
Note: Before starting a real-time run, the instrument may pause (up to
15 minutes) to heat the heated cover to the appropriate temperature.
If using the SDS Enterprise Database, the Automation Controller Software
automatically conducts a primary analysis of each completed run and saves the
results to the database. If you specified a study or studies as explained in
"Associating Session and Study Data" on page
automatically attaches the analyzed session data to the appropriate study.
The following options are available during and after the completion of the run:
To...
Monitor the progress of the run
Stop the run
IMPORTANT!
If you must stop a run in-progress for any reason,
carefully read the instructions on
Open the instrument tray (after the run)
Analyze the run data after the run is complete
Running Plates Using the Automation Controller Software
from the Plate Queue tab.
4-33, then the software
page 4-28 before halting the run.
DRAFT
September 1, 2004 11:39 am, CH_Basic.fm
See Page
4-44
4-44
4-44
4-44
4-43