Well-Marker Information; Well-Marker List Header (Allelic Discrimination Only; Well-Marker Definition List (Allelic Discrimination Only; Setup Table Files - Applied Biosystems 7900HT User Manual

Appendix A Software Reference

Well-Marker Information

12. Well-Marker List Header (Allelic Discrimination Only)

Description
Format
Example
Example Code A-11
Well Sample Name

13. Well-Marker Definition List (Allelic Discrimination Only)

Description
A-12 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
September 1, 2004 11:39 am, App_SWReference.fm
Element numbers 12 and 13 define the contents of the wells on the plate in terms of
markers. The well-marker information consists of two sections: the Well-Marker List
Header and the Well-Marker Definition List.
Well-Marker List Header information is required only in setup table
IMPORTANT!
files created for allelic discrimination runs. This section does not appear in setup
table files created for absolute or relative quantification runs.
This line contains the column headings for the well-marker information section of the
setup table file that make the file easier to edit using a program such as Microsoft Excel.
[ Well <tab> Sample Name <tab> Detector <tab> Task <tab> Quantity ] {
... <tab> Detector <tab> Task <tab> Quantity } [ <cr> ]
Marker Task
Well-Marker Definition List information is required only in setup
IMPORTANT!
table files created for allelic discrimination runs. This section does not appear in
setup table files created for absolute or relative quantification runs.
This section defines the contents of the plate wells. The setup table file must contain a
definition for each well used on the plate. Each well definition list consists of one string
of characters terminated by a <cr>. The definition consists of three main divisions:
• Well number – The first tab-delimited text block defines the number of the well on
the plate. Well numbers start at 1 for well A-1 (upper-left corner of the plate) and
increases from left to right and from top to bottom. The wells must be listed in
order (1,2,3,...).
• Sample name – The second text block defines the name of the sample assigned to
the well.
• Marker assignments – The remaining tab-delimited text blocks for the well
definition define the marker assigned to the well. Each marker is represented by
two text blocks that define the following information:
– The name of the marker
– The task assignment of the marker for the well (UNKN - Unknown, NTC - No
Template Control)
To assign more than one marker to a well, repeat the marker definition text blocks for
each detector. There is no limit to the number of markers that can appear in a well.
All markers that appear in this section must have been previously
IMPORTANT!
defined in the Marker Definitions section (elements 7–9).
DRAFT
Marker Task