Applied Biosystems 7900HT User Manual page 376

relative quantification
automatic outlier removal
Automation Accessory
components 7-36
Plate Handler, See Plate Handler
See fixed-position bar code reader
Automation Controller Software
about 4-39
adding plates to the plate queue
configuring for operation
ejecting a plate 4-44
launching 4-39
monitoring instrument progress
removing plates from the plate queue
starting the plate queue
stopping the plate queue
B
background run 7-16 to
about 7-16
constructing a background plate
creating a plate document
extracting 7-19
preparing a background plate
troubleshooting 8-9
when to perform 7-16
bar code information
entering into a plate document 2-27,
bar code readers
fixed position, See fixed-position bar code reader
hand held, See hand-held bar code reader
bars, in the Gene Expression Profile
symbols 6-31
baseline
about D-6
configuring value for automatic analysis
Block readout (from the Real Time tab)
Bucket Type, setting on centrifuge
C
calibrating the 7900HT instrument
adjusting the plate-sensor switch
aligning the fixed-position bar code reader
aligning the Plate Handler
performing background run
performing pure dye (spectral) run
calibrator sample 6-20
Centrifuge system
bucket requirements
Bucket Type, setting
components 4-12
installation and operation
placing bucket in centrifuge
requirements 4-12
TaqMan Low Density Array, placing in
Index-2 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
September 1, 2004 11:38 am, 4351684AIX.fm
B-6
6-19
4-40
4-42
4-44
4-40
4-43
4-44
7-19
7-17
7-17
7-17
3-28
6-11
4-27
4-13
7-37
7-49
7-41
7-16
7-20
4-16
4-13
4-13
4-17
DRAFT
bucket 4-16
Centrifuging, TaqMan Low Density Array
changing
gripper finger pads
pane, view, and plot sizes
sample block module
checklists
absolute quantification
allelic discrimination
dissociation curve 6-22,
relative quantification
chemistry
5´ Nuclease (TaqMan)
non-optimized 8-6
SYBR Green D-3
troubleshooting 8-5
cleaning
gripper finger pads
sample block wells
closing
instrument tray (Automation Controller
Software)
instrument tray (SDS software)
plate documents 2-18
Zymark Twister Software
comments
adding to a detector
adding to a plate document
Comparative C Method
T
about 6-4, D-8
formula derivation
Components
centrifuge system 4-12
TaqMan Low Density Array
computer
about 1-6
hard drive partitions
maintaining 7-54
minimum system requirements
troubleshooting 8-14
turning ON 2-4
configuring the 7900HT instrument for 9600
emulation 3-21
consumables
384-Well Optical Reaction Plates
96-Well Optical Reaction Plates
improper or damaged plastics
Optical 96-Well Fast Thermal Cycling Plates
Optical Adhesive Covers
Optical Cap Strips
writing on reaction plates
Contamination
genomic DNA 4-11
preventing 4-6
contamination
decontaminating the sample block
4-16
7-52
2-21
7-6
6-9
5-10
6-40
6-22
D-2
to 8-8
7-52
7-15
4-44
4-29
7-48
3-11
3-28
D-8
4-10
1-6
1-6
C-3
C-4
8-8
C-3
C-3
8-7
7-14
C-4