Applied Biosystems 7900HT User Manual page 127

Laboratory Setup
Setting the
Bucket Type for
the Low Density
Array
.
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
The centrifuge system is designed to be placed on a standard laboratory bench. It
requires access to 115 VAC, 60 Hz electrical power. Consult the owner's manual
should for specific operating and site preparation instructions.
1. Power on the centrifuge.
2. Following the instructions in the Sorvall Legend T Centrifuge operator's
manual, use the front panel controls on the centrifuge to set the bucket type for
the centrifuge to 15679
Be sure to set the correct bucket type. Setting the correct bucket
IMPORTANT!
type ensures that the maximum rotational speed stays within the manufacturer's
specified limits.
3. Using the front panel controls, set the following operational parameters:
Parameter
Up ramp rate
Down ramp rate
Rotational speed
Centrifugation time
Buttons 1–4 are
programming
buttons.
Up ramp
rate control
4. Power off the centrifuge.
Preparing TaqMan Low Density Arrays for Use
Value
9
9
1200 rpm (331 × g)
1 min
rpm indicator
Down ramp rpm
rate control controls
DRAFT
September 1, 2004 11:39 am, CH_Basic.fm
Bucket type control
Scrolling control
Time
controls
4-13