Operating The Software With An Sds Enterprise Database - Applied Biosystems 7900HT User Manual

Chapter 4 Operating the Instrument

Operating the Software with an SDS Enterprise Database

Overview
Workflow
Logging into the
Automation
Controller
Software
4-32 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
September 1, 2004 11:39 am, CH_Basic.fm
Running the Automation Controller Software with the SDS Enterprise Database is
the least complicated option for operating the Applied Biosystems 7900HT Fast
Real-Time PCR System. Plate documents saved to the SDS Enterprise Database are
automatically queued (set up for operation) for use on the Automation Controller
Software. Following plate document creation, plates can be prepared and loaded
immediately onto the Zymark Twister Microplate Handler for use.
1. Start the Automation Controller Software and log in to the database (see
page 4-32).
2. If desired, set the study associations for the relative quantification and allelic
discrimination runs (see
3. Load prepared optical plates and Low Density Arrays onto the stacks of the
Zymark Twister Microplate Handler (see
4. Run the instrument (see
5. Analyze the run data.
– Allelic Discrimination Analysis (see
– Absolute Quantification Analysis (see
– Relative Quantification Analysis (see
– Dissociation Curve Analysis (see
1. If running the SDS software, do one of the following:
• Select Instrument > Disconnect to discontinue communication between
the software and the instrument, or
• Select File > Exit to close the SDS software.
2. Start the Automation Controller Software
(select > Programs >
Automation Controller 2.2).
3. In the Login dialog box, enter your user name and password, then click
to log into the database.
4. Specify the Session-Study associations as described in
Study Data" on page
5. Prepare and load the plates onto the Plate Handler as explained in
Plates onto the Plate Handler" on page
DRAFT
page 4-33).
page 4-41).
page 4-43).
page 5-5).
page 6-5).
page 6-15).
page 6-37).
Applied Biosystems >
4-33.
4-41.
SDS 2.2.1 >
"Associating Session and
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