Applied Biosystems 7900HT User Manual page 379

adjusting step parameters
configuring data collection options
programming 3-20
removing a step 3-21
setting the sample volume
minimum
gene expression level
modes of operation 1-17
automated 1-18
database 1-18
stand-alone 1-17
mRNA targets, number per well
N
no template control (NTC)
detector task 3-16
NTC calls applying
NTC calls verifying
Number of targets per well
O
↓
6-33
opening
instrument tray (Automation Controller
Software)
instrument tray (SDS software)
plate documents 2-19
operating system
supported 1-6
upgrading 7-55
operating the 7900HT instrument
power switch 2-4
optimizing primer and probe concentrations
outliers
allelic discrimination
automatic outlier removal
outlying amplification
multi-reporter experiments
P
panes
hiding 2-21
maximizing/minimizing
resizing 2-21, 2-22
showing 2-21
passive reference
setting 3-18
PCR
5´ Nuclease Assay
Geometric (Exponential) Phase
kinetic analysis of D-4
Linear Phase D-5
Plateau Phase D-5
SYBR Green Chemistry
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
3-21
3-22
to 3-23
3-22
6-33
4-10
5-15
5-16
4-10
4-44
4-29
B-3
5-8
6-19
6-19
2-22
D-2
D-4
D-3
PCR step
fill reservoirs, checking after centrifuging
fill reservoirs, trimming after sealing
general practices
placing in bucket in centrifuge
TaqMan Low Density Array, centrifuging
TaqMan Low Density Array, loading
TaqMan Low Density Array, placing in
bucket
TaqMan Low Density Array, sealing
persisted data
analysis sessions
plate documents
1-28
studies 1-28
pipetting errors 8-6
pipettors, using 8-6
plate adapter, changing
plate document information
applying to a plate document
plate documents 3-9
about 2-12
adding to the plate queue 4-35,
applying detector tasks
applying sample names
assigning standard quantities
closing 2-18
configuring document information
copying detectors
copying markers
creating 2-13, 3-9
creating from a template 3-26,
exporting 2-14
importing setup data 2-16,
methods, See methods
opening 2-19
programming methods
removing from the plate queue
running batches
running individually
saving 2-17
saving as a single plate file
saving as a template file
saving to the database
setting Sample Volume
setting the passive reference
plate grid
about 2-29
selecting wells 2-24
viewing well information
zooming 2-26
Plate Handler 1-8, 7-36
adjustment knob
aligning 7-41 to
cleaning the finger pads
gripper 7-36
plate stack positions
DRAFT
September 1, 2004 11:38 am, 4351684AIX.fm
4-21
4-6
4-17
4-14
4-16
4-19
1-28
7-12
3-28
4-40
3-16
3-28
3-17
3-28
3-13
3-15
4-36
A-2
3-19
4-40
4-39
3-29
4-24
3-24
4-24
3-22
3-18
2-23
7-36
7-48
7-52
7-36
4-18
4-16
Index-5