Step 3 - Configuring The Plate Document With Tasks - Applied Biosystems 7900HT User Manual

Chapter 3 Preparing a Run
Step 3 – Configuring the Plate Document with Tasks
User Access
Requirement
About Detector
Tasks
Applying
Detector Tasks
Selected wells
(task applied
to selection)
3-16 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
September 1, 2004 11:39 am, CH_Run.fm
If using the SDS Enterprise Database, you must belong to the Scientist or
Administrator User Group to apply or modify detector task settings.
You must assign a 'task' to the detectors or markers applied to each well of the plate
document that defines their specific purpose or function on the plate. The SDS
software uses the detector task assignments to determine how to treat the data
produced by the wells when analyzing the run data. Detector tasks vary depending on
the type plate document.
1. Using the Ctrl and Shift keys, select the wells of the plate grid containing
samples for a particular task described in the table below.
Table 3-1 Detector Tasks of the SDS Software
Experiment
Allelic Unknown
Discrimination
NTC
Absolute Unknown
Quantification
Standard
NTC
Relative Target
Quantification
Endogenous
Control
2. In the well inspector, click the field in the Task column for each detector entry,
and select the appropriate task from the drop-down list. The SDS software labels
all selected wells with the task.
DRAFT
Task Apply to...
all detectors of wells that contain PCR reagents
and test samples.
all detectors of negative control wells that contain
reagents for the PCR, but lack template.
all detectors of wells containing PCR reagents and
test samples for quantification.
the appropriate detectors of wells that contain
PCR reagents and samples of known quantities.
(See page 3-17
all detectors of negative control wells that contain
PCR reagents, but lack template.
all detectors of wells that contain PCR reagents for
the amplification of target sequences.
all detectors of wells that contain reagents for the
amplification of the endogenous control sequence.
for instructions.)
Task drop-down list