Step 4 - Setting The Passive Reference And Omitting Wells - Applied Biosystems 7900HT User Manual

Chapter 3 Preparing a Run
Step 4 – Setting the Passive Reference and Omitting Wells
User Access
Requirement
Setting
the Passive
Reference
Omitting Wells
from Use
Selected wells
(removed from use)
3-18 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
September 1, 2004 11:39 am, CH_Run.fm
If using the SDS Enterprise Database, you must belong to the Scientist or
Administrator User Group to change the passive reference or omit wells from use.
If using an Applied Biosystems chemistry, use the default Passive Reference setting
(Applied Biosystems chemistries use the ROX
running a custom chemistry, select a dye to use as a passive reference for the run.
Applied Biosystems recommends using a passive reference to normalize the
Note:
signals from the reporter dyes.
1. In the Passive Reference drop-down list, select the appropriate reference dye.
2. If necessary, omit wells from use as explained below. Otherwise, program the
method for the run as explained on
The SDS software allows you to omit wells from use before or after running a plate.
If you remove a well removed from use before you run a plate
IMPORTANT!
document, the SDS software will not collect data for the well during the run.
However, if you remove a well from use after you run a plate document, the software
excludes the well from the analysis but does not delete the data.
1. While pressing and holding the Ctrl key, select the well(s) in the grid that you
want to remove.
2. In the well inspector of the Setup tab, click the Omit Well(s) check box.
3. Program the method for the run as explained on
DRAFT
™ passive reference dye molecule). If
Passive Reference drop-down list
Select the appropriate
passive reference
page 3-19.
Omit Well(s) check box
(selected)
page 3-19.