After The Run - Applied Biosystems 7900HT User Manual

Chapter 4 Operating the Instrument
Monitoring
Instrument
Progress
Stopping the
Instrument
Ejecting a Plate

After the Run

Analyzing the
Run Data
4-44 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
September 1, 2004 11:39 am, CH_Basic.fm
The Automation Controller Software displays the progress of the current run in the
Thermal Status tab. See
To stop the plate queue, click
box at any time.
If you clicked
while the Plate Handler is
handling a plate
after a plate has been loaded
into the instrument, but
before the run has started.
after the 7900HT instrument
has started a run.
To eject a plate following a halted run (or to open/close the instrument tray),
click from the Automation Controller Software window.
You can analyze the run data immediately following the completion of the run.
See the appropriate page for your application:
Section 5.1 Allelic Discrimination. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-5
Section 6.1 Absolute Quantification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-5
Section 6.2 Relative Quantification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-15
Section 6.3 Dissociation Curve Analysis. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-37
DRAFT
page 4-27 for an explanation of the Thermal Status tab.
in the Automation Controller Software dialog
... Then the instrument...
aborts the run and moves the Plate Handler to the home
position.
aborts the current run, ejects the plate, and moves the
Plate Handler to the home position.
aborts the current run, ejects the plate, and moves the
Plate Handler to the home position.
IMPORTANT!
Stopping a run during thermal cycling can
affect the chemistry of the reactions inside the plate.
Before stopping a run, carefully read the guidelines on
page 4-28 to determine the best course of action.