Analyzing The Background Data - Applied Biosystems 7900HT User Manual

Analyzing the Background Data

Extracting the
Background
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
2. Click .
The 7900HT instrument begins the background run.
Before starting the run, the instrument may pause (up to 15 min) to heat
Note:
the heated cover to the appropriate temperature.
3. When the background run is complete and the Run Complete dialog box
appears:
a. Click
b. Click
c. Extract the background component as explained on
In this procedure, you will extract the calibration values from the background plate
document. After extraction, the SDS software stores the data as part of the calibration
file located in the Calibration subdirectory of the SDS directory.
1. Select Analysis > Extract Background.
The software attempts to extract the background signal and displays the success
of the extraction in a dialog box.
If the software displays the:
• Calibration Update Complete dialog box, then the run is successful.
Proceed to step
The raw spectra read from the Background plate conform to acceptable
limits.
• Error dialog box, then the run is unsuccessful. Troubleshoot and
decontaminate the sample block as explained in
page 8-9.
The software has stopped the extraction because one or more raw spectra
exceed 2500 FSU.
2. Click (or select File > Save).
The software saves the plate document.
3. Select File > Close.
The software closes the plate document.
to close the dialog box.
and remove the plate from the instrument tray.
2.
DRAFT
September 1, 2004 11:39 am, CH_Maintenance.fm
Performing a Background Run
page 7-19.
"Background Runs" on
7-19