Taqman Low Density Array - Applied Biosystems 7900HT User Manual

Chapter 8 Troubleshooting

TaqMan Low Density Array

Low Density Array
Troubleshooting
Table
8-18 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
September 1, 2004 11:39 am, CH_Trouble.fm
Table 8-6 Troubleshooting the TaqMan
Observation
After removing the Low
Density Array from its
packaging, the fill
consumable is damaged
(creased, bent, or folded).
After removing the Low
Density Array from its
packaging, the aluminum
foil backing is damaged
(creased, bent, or folded).
After removing the Low
Density Array from its
packaging, dust or other
particulates settle on the
reaction wells (optical side
of the Low Density Array).
After removing the Low
Density Array from its
packaging, water
condenses on the reaction
wells (optical side of the
Low Density Array).
DRAFT
® Low Density Array
Possible Cause
N/A
N/A
N/A
The Low Density Array may
not have come to room
temperature before being
removed from its
packaging.
Recommended Action
If the fill consumable is
damaged, then the PCR
reaction mixture might not
flow into the reaction wells
during centrifugation.
Discard the Low Density
Array.
If the aluminum foil backing
is damaged, then the PCR
reaction mixture might not
flow into the reaction wells
during centrifugation.
Discard the Low Density
Array.
Remove dust or particulates
by lightly tapping or blowing
on the reaction wells.
Room-temperature,
pressurized nitrogen or an
air blower may be used.
IMPORTANT!
Be sure to
remove all dust and
particulates. The reaction
wells (optical side of the
Low Density Array) must be
free of dust and
particulates, (especially
fluorescent particulates)
Remove condensation by
lightly blowing on the
reaction wells.
Room-temperature,
pressurized nitrogen or an
air blower may be used.
IMPORTANT!
Be sure to
remove all water
condensation. The exterior
surface of the reaction wells
(optical side of the Low
Density Array) must be free
of water condensation.