Applied Biosystems 7900HT User Manual page 380

plate-sensor switch
replacing the finger pads
turning ON 2-4
plate queue
adding plate documents (Automation Controller
Software)
adding plate documents (SDS software)
adding plate documents (Template Batch
utility) 4-36
removing plate documents
starting 4-43
stopping 4-44
plate stacks
loading plates 4-41,
placing in use 4-42
positions 7-36
Plateau Phase of the amplification curve
plates
running batches 4-39,
running individually
types, See consumables
plate-sensor switch 7-36
adjusting 7-37 to 7-40
plots
hiding 2-21
maximizing/minimizing
resizing 2-21, 2-22
showing 2-21
Port Number A-22
Post readout (from the Plate Read tab)
Pre readout (from the Plate Read tab)
precision
causes of low precision
preparing
master mixes B-3
plate for a run 4-6
primer and probe concentrations
optimizing B-3
programming
methods (absolute quantification)
methods (allelic discrimination)
methods (dissociation curve analysis)
methods (relative quantification)
temperature ramp 3-23
Protocol
preventing contamination
pure dye plate
constructing for custom dyes
preparing for use 7-24
pure dye run
about 7-20
creating a plate document
extracting data 7-25
performing 7-24
troubleshooting 8-11
when to perform 7-20
Index-6 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
September 1, 2004 11:38 am, 4351684AIX.fm
7-36
7-52
4-40
4-35
4-40
4-42
D-5
4-41
4-25
2-22
4-27
4-27
8-5 to 8-8
3-21
3-20
3-23
3-21
4-6
7-27
7-21
DRAFT
Q
Quality control, TaqMan Low Density Array
quantifying
probes and primers
standards for absolute quantification
quantitative RT-PCR
about 6-4
absolute, See absolute quantification
relative, See relative quantification
types of 6-4
quantities, applying to a plate document
R
R2 readout (from the Standard Curve Plot)
reagents
custom oligonucleotides
non-Applied Biosystems PCR reagents
TaqMan Pre-Developed Assays and Reagents
TaqMan RNase P Instrument Verification
Plates C-5
re-connecting the SDS software
relative quantification
about 6-4, 6-16 to 6-20
analyzing data 6-22
assay development guidelines
procedure checklist
the Comparative C Method
T
the Standard Curve Method
troubleshooting 8-12
removing
plate documents from the plate queue
steps from a method
Rep readout (from the Real Time tab)
replacing
gripper finger pads
sample block module
replicate wells, use of 6-20
reproducible limits 6-19
resizing panes, views, and plots
restacking plates 4-42
restoring
database connection
RQ Manager Software
RQ Min/Max Confidence
running
batches of plates 4-39,
single plate 4-25
S
sample
calibrator 6-20
sample bars
about 6-31
4-12
B-3
B-6
3-17
6-13
C-6
8-8
4-30
to 6-35
B-6
6-22
6-4
6-4
4-40
3-21
4-27
7-52
7-6
2-21
A-22
1-25
6-28
4-41
C-6