Applied Biosystems 7900HT User Manual page 370

detector
endogenous
control
endogenous
reference
end-point
(plate read)
run/analysis
expression fold
value
hands-off
automation
high throughput
(HT)
hold
locus
marker
method
Glossary-4 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
September 1, 2004 11:39 am, Glossary.fm
A virtual representation of a gene- or allele-specific nucleic acid probe reagent used
for absolute quantification, relative quantification, allelic discrimination, or other
analyses performed on an SDS instrument. Examples of reagents represented as
detectors include TaqMan probes and the SYBR Green 1 dsDNA Binding Dye.
Detector for a gene presented at a consistent level in all experimental samples. By
using an endogenous control as an active reference, one can normalize quantification
of a messenger RNA (mRNA) target for differences in the amount of total RNA added
to each reaction.
See endogenous control.
Refers to processing of samples for allelic discrimination and plus/minus scoring
chemistries of samples by the 7900HT instrument. The 7900HT instrument collects a
single reading after prior PCR temperature cycles are completed using a thermal
cycler. After analysis by the SDS software, the resulting multi-component data is used
to assess the presence of target sequences in the unknown samples.
The expression of a target gene relative to control gene in one sample, relative to the
expression level in a calibrator sample. See
After plates have been loaded into the Zymark
and the Automation Controller Software has been told to start running, no human
intervention should normally be required until all plates in the robot stacks have been
processed. With the SDS Enterprise Database option, this includes plate document
analysis and analysis results persistence to the database.
Describes the ability to analyze a large quantity of samples for genotyping or gene
expression analysis.
A thermal cycling set-point of a method that maintains a specified temperature for an
extended period of time.
The physical location of a site on a chromosome, sometimes understood as the site of
a gene, usually understood as the site of a marker variant.
For SDS software, a virtual representation of a variable segment of DNA (such as a
SNP) whose inheritance can be followed, comprising a name and a set of two detectors
specific for the allelic variants. Markers are used only for allelic discrimination
assays, not for absolute quantification, or relative quantification. Allelic
discrimination plate documents must contain at least one marker. The marker must be
configured with two detectors before it can be applied to a plate document.
The protocol that the Applied Biosystems 7900HT Fast Real-Time PCR System
follows during a real-time or end-point sequence detection run. Methods used by the
7900HT instrument make use of the following information: thermal cycling times and
temperatures, ramp rates, auto-increment values, and data collection settings.
DRAFT
∆∆
C .
T
® Twister Microplate Handler stacks,