Chapter 1 Product Overview; System Overview - Applied Biosystems 7900HT User Manual

Chapter 1 Product Overview

System Overview

About the
7900HT
Instrument
Supported Runs
and Chemistries
1-2 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
September 1, 2004 11:38 am, CH_Overview.fm
The Applied Biosystems 7900HT Fast Real-Time PCR System is a
second-generation sequence detection system instrument designed for automated,
high-throughput detection of fluorescent PCR-related chemistries. The instrument is
capable of real-time, end-point, and dissociation curve analysis of assays arrayed on
multiple formats. The 7900HT instrument is optimized for use with
Applied Biosystems chemistries for nucleic acid quantification and detection.
Figure 1-1 The Applied Biosystems 7900HT Fast Real-Time PCR System with
the Automation Accessory
The Applied Biosystems 7900HT Fast Real-Time PCR System provides two types of
runs to support the variety of PCR-related chemistries available from
Applied Biosystems and affiliated companies.
End-Point (Plate Read) Chemistry
In an end-point run, the thermal cycling of prepared plates containing reagents and
template is performed on a dedicated thermal cycler. Following the PCR, the 7900HT
instrument is then used to collect a plate-read or a single reading of the fluorescence
resulting from the completed reactions. Allelic Discrimination is an example of an
end-point chemistry that is directly supported by the SDS software.
Real-Time Chemistry
In a real-time run, the thermal cycling of prepared plates containing reagents and
template is performed on the 7900HT instrument. During the run, the instrument
collects data at each cycle of the PCR. The resulting data provides a chronological
series of measurements of the fluorescence resulting from the reactions. Examples of
real-time chemistry include absolute quantification, relative quantification and
dissociation curve analysis.
DRAFT