Applied Biosystems 7900HT User Manual page 205

Replicate and
Outlier Removal
for Study Settings
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
• Manual Ct – Instructs the software to use the baseline and threshold values
specified in the Analysis Settings dialog box to calculate C
detectors.
The software provides two methods for manually setting the baseline and
threshold values for the detector:
– If you know the values that you want to use, enter them into the appropriate
fields of the Analysis Settings dialog box.
– If you want to set the baseline and threshold using the controls of the
Amplification Plot tab, leave the fields empty and set the values later as
described on page
The settings of this group box determine how the software identifies and removes
outlier data for all detectors in the study.
Figure 6-10 Replicate and Outlier Removal for Study Settings
• Automatic Outlier Removal – Instructs the software to automatically remove
the data of outlying replicate wells using the outlier removal algorithm. See
"Automatic Outlier Removal" on page 6-19
• Omit replicates whose ∆C
analysis, the data of any sample well whose ∆C
cycles specified in the associated field. This option is only available for
multiplex experiments where similar target and endogenous control C
indicate that competition of the assays is affecting the amplification.
You can also identify and eliminate outliers manually, as explained in
Note:
"Eliminating Outlying Amplification" on page
6-46.
Automatic Outlier
Removal check box
Omit replicates whose ∆C
check box
for an explanation of the algorithm.
<=... – Instructs the software to remove from the
T
6-49.
DRAFT
September 1, 2004 11:39 am, CH_Real-Time.fm
Analyzing the Study
s for the selected
T
<=
...
T
is less than the number of
T
T
s may
6-27