Applied Biosystems 7900HT User Manual page 206

Chapter 6 Analyzing Real-Time Data
Gene Expression
Settings for Study
Settings
6-28 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
September 1, 2004 11:39 am, CH_Real-Time.fm
The settings in this group box determine how the software handles the endogenous
control data and RQ Min/Max confidence values for your study. These settings are
crucial to the analysis and are set once for the study.
Figure 6-11 Gene Expression Settings for Study Settings
• Calibrator Sample – Displays the name of the sample that the software uses as
the calibrator for the study.
• Endogenous Control Detector – Specifies the name of the detector that the
software will use as the endogenous control for the study.
• Control Type – The Multiplexed/Non-Multiplexed option buttons specify how
the software will use the data from the wells labeled with the endogenous
control data.
• RQ Min/Max Confidence – Specifies the confidence value that the software
will use to calculate the standard error of the mean expression level (RQMax
and RQMin values) for the samples in the study.
• Export Individual ∆C
individual samples in the analysis instead of the average ∆C
replicate group.
DRAFT
Calibrator Sample drop-down list
Endogenous Control Detector
drop-down list
Control Type option buttons
RQ Min/Max Confidence drop-down list
s – Instructs the software to export the ∆C
T
values of
T
values for the
T