Set Up The Run Method - Applied Biosystems 7500 Getting Started

Chapter 2 Design the Standard Curve Experiment

Set Up the Run Method

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Information

Set Up the Run Method

About the
Example
Experiment
Notes
30
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Standard Curve
Set Up Biological Replicate Groups
1. Select Specify Biological Replicate Groups.
2. Enter the number of biological replicate groups that you want to test in the reaction
plate.
3. For each biological replicate group, click the cell in the Biological Group Name
column, then enter a name for the biological group. For example, BrainGP.
4. Assign the biological replicate group(s) to the reaction plate:
Select wells in the reaction plate that contain samples associated with the
a.
biological replicate group.
In the Assign column, select the check box of the appropriate biological
b.
replicate group.
4b
3
For more information on:
• Completing the Samples screen – Open the 7500 Software Help by clicking
pressing F1.
• Using Advanced Setup – See
In the Run Method screen, review the reaction volume and the thermal profile for the
default run method. If needed, you can edit the default run method or replace it with one
from the Run Method library.
In the standard curve example experiment, the default run method is used with one
change: The reaction volume per well is changed from 50 µL to 25 µL.
"Advanced Setup Workflow" on page
1
2
or
98.
Experiments