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Chapter 2 Operate the Automated Thermal Cycler (ATC) System as a stand-alone system
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Program and execute a run on the ATC
Program and execute a run on the ATC
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Figure 10 Run Protocol
Add (+), Remove (−) stages
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Remove and arrange order
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Run Mode drop down
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Cover Temperature and Sample Volume
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Temperature, Ramp Rate, Hold Time
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Cancel
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Start Run
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Export SiLA
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Save
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Load
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1. In Stages, add or remove protocol stages with the plus and minus signs.
2. Click up and down arrows to arrange the sequence of stages.
Specify the number of cycles required for the particular stage.
3. In the Steps field, specify the temperature set points, ramp rate, and duration.
The ramp rate is indicated as a % where 100% corresponds to a peak block ramp rate of 3.5C/s.
Type in −1 in the Hold Time field to achieve an infinity hold time.
4. Enter cover temperature and sample volume.
5. Click Start Run to start immediately, or Save for future use.
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Automated Thermal Cycler (ATC) System User Guide
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