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Run the RNase P
plate or array card
QuantStudio
6 and 7 Flex Real-Time PCR Systems v1.6 Maintenance and Administration Guide
™
d. Dispense the fluid so that it sweeps in and around the fill reservoir toward
the vent port. Pipet the entire 100 µL into the reservoir, but do not go past the
first stop of pipettor plunger or you may blow the solution out of the port.
IMPORTANT! Do not allow the tip to contact and possibly damage the
coated foil beneath the fill port.
5. Centrifuge and seal the array card as explained in step 6 through step 8 in "Fill
the calibration TaqMan
6. Run the prepared array card as soon as possible after filling it.
IMPORTANT! Store the array card in a dark place until you are ready to run it. The
fluorescent dyes in the array card are photosensitive. Prolonged exposure to light can
diminish the fluorescence of the dye.
1. In the QuantStudio
Console.
2. In the Instrument Console, select the icon for your instrument, then click
Manage Instrument.
Note: If the Manage Instrument button is inactive, add your system to the My
Instruments group.
3. In the Instrument Manager, start the RNase P run:
a. Click
Maintenance, then click RNase P Run.
b. In the RNase P Run screen, click Start RNase P Run.
4. Click Next, then prepare for the instrument verification test as instructed.
5. At the bottom of the Setup tab, enter the reagent information for the RNase P
plate or array card that you are using.
Array Cards" on page 37.
®
™
Real-Time PCR Software Home tab, click Instrument
Chapter 2 Calibrate the instrument
Verify the instrument performance
2
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