Using Conc/Factor To Measure Concentration - Thermo Scientific Orion AquaMate 8000 UV-Vis User Manual

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Using Conc/Factor to Measure Concentration

Thermo Scientific
2. Press Measure Blank.
If the Single Cell Changer is installed, remove the blank and place the standard in the cell
changer.
3. Press Units/Standard to set the units and measure the standard.
4. Press Enter Conc, enter the concentration value of the standard and press Enter.
5. Press Select Units, highlight the appropriate unit in the list and press Enter.
6. Press Measure Standard.
The instrument measures the absorbance of the standard and displays the absorbance and
calculated factor.
In this mode a concentration factor is used to determine the concentration of samples. The
concentration of unknown samples is determined ratiometrically from the entered factor. The
measurement can be expressed mathematically as
(Abs / %T of Sample) * Factor = Concentration of Sample
where the factor is entered, the Abs/%T of the sample is measured, and the sample
concentration is calculated.
To use Conc/Factor to measure concentration
1. If necessary, press Change Mode to switch to the Conc With Factor mode.
2. Press Units/Factor to set the factor and select the units.
3. Press Enter Factor.
4. Type the desired factor value.
13
Basic Concentration Measurements—Basic A-%T-C
Using Conc/Factor to Measure Concentration
Orion AquaMate 7000 Vis and Orion AquaMate 8000 UV-Vis
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