Instrument Description; A Look At The Sample Block - Applied Biosystems GeneAmp PCR System 9600 User Manual

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restriction sites. Dideoxy sequencing can also be performed on
the amplified product.

Instrument Description

The GeneAmp PCR System 9600 contains a programmable
heating and cooling block, which rapidly and uniformly heats
and cools PCR samples.

A Look at the Sample Block

The sample block is a 96-well aluminum block. Up to 96 samples
in MicroAmp Reaction Tubes (0.2 mL) are loaded in a sample
tray and placed in the block. When a tray is in place, the heated
cover is moved forward over the sample block area and exerts an
even pressure on all tubes.
Figure 1-2 Sample block and heated cover.
There are 17 holes within the block for coolant flow: eight of the
holes are used for rapid sample block cooling (ramp cooling) and
the remaining nine are for cold-biasing the system. A Kapton
heater beneath the sample block is responsible for rapid heating
and control of the sample block. The heater is designed so that
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Sample Block
Heated Cover
1-3

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