Creating A Plate Document For Sample Amplification; Chapter 5 Generating Amplification Data - Applied Biosystems 7300 Getting Started Manual

Chapter 5 Generating Amplification Data

STANDARD STANDARD STANDARD

Creating a Plate Document for Sample Amplification

Creating a Plate Document for Sample Amplification
Benefits of
Real-Time
Amplification
Using AQ Plate
Documents for
Amplification
Detector Tasks
Notes
28
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Plus/Minus Assay Getting Started Guide
Because the plus/minus assay is an end-point assay, you can amplify the target sequences
offline using any thermal cycler. However, using the 7300/7500/7500 Fast system to
amplify the target sequences provides real-time PCR data. After the plus/minus samples
are analyzed, you can study the amplification plots if you observe questionable calls or
observe no data for a well.
You create and use absolute quantification (AQ) plate documents to store real-time data
for plus/minus assays. Because the AQ plate document is used only to amplify target
sequences (not to quantify the PCR data), you do not need a standard curve for the AQ
plate.
For AQ plate documents, there are three types of tasks:
Task
Unknown
Standard
NTC
The task label "unknown" is used for both IPC and the target samples.
Symbol
Detectors of wells that contain target sequences.
Detectors of wells that contain samples of known
quantities.
Detectors of negative control wells that contain no
template.
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