Decontaminating The Sample Block - Applied Biosystems 7300 Installation And Maintenance Manual

Appendix A

Decontaminating the Sample Block

Decontaminating the Sample Block

Time Required
Materials
Required
Notes
108
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
PHYSICAL INJURY HAZARD. Do not remove the instrument
cover. There are no components inside the 7300/7500/7500 Fast system that you can
safely service yourself. If you suspect a problem, contact an Applied Biosystems Service
Representative.
PHYSICAL INJURY HAZARD. During instrument operation, the
sample block can be heated to 100 °C. Before performing the following procedure, be
sure to wait until the sample block reaches room temperature.
The following procedure explains how to eliminate fluorescent contaminants from the
sample block of the 7300/7500/7500 Fast instrument. Perform the procedure to resolve
problematic background runs where one or more wells consistently exhibit abnormally
high signals, indicating the presence of a fluorescent contaminant.
30 minutes
95% ethanol
Pipette (100-µL)
solution
with pipette tips
Deionized
water
10% bleach
solution
Cotton or nylon
Safety
swabs and lint-
glasses
free cloths
Screwdriver
Powder-free
gloves