Creating An Rq Study Document; Chapter 6 Analyzing Data In An Rq Study - Applied Biosystems 7500 Getting Started Manual

Chapter 6 Analyzing Data in an RQ Study

Creating an RQ Study Document

Creating an RQ Study Document
Notes
60
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Relative Quantification Getting Started Guide
To conduct a comparative analysis of RQ plates in a study or to analyze a single RQ
plate experiment, you must first create an RQ Study document.
RQ Study software is an optional package for the 7300 instrument, but it
IMPORTANT!
is standard for the 7500 and 7500 Fast instruments.
RQ plates may be run with either standard or Fast thermal cycling
IMPORTANT!
conditions. An RQ study must be composed of RQ plates of the same thermal cycling
protocol. RQ plates run on standard thermal cycling protocols and RQ plates run on Fast
thermal cycling protocols cannot be combined into a single RQ study.
The SDS Software 1.3.1 uses the comparative Ct method
− ∆∆Ct
(2 ) of relative quantification. For more information about methods of calculating
relative quantification, refer to ABI P
Bulletin #2 (PN 4303859).
In an RQ study, you can...
• Select the endogenous control and the
calibrator sample.
• Select the control type when applicable.
• Set baseline and threshold values and RQ
Min/Max Confidence Levels.
• Omit individual wells or sample replicates.
®
7700 Sequence Detection System User
RISM
You cannot...
• Create, add, or modify samples.
• Create, add, or modify detectors.
• Change detector tasks.
(You can perform these operations in RQ
Plate documents.)