Nucleic Acid Labels - Thermo Scientific Evolution 200 Series User Manual

Nucleic Acid Labels

Thermo Scientific
5. Enter the molecular weights of any additions to the base sequence in the Modification
box.
The calculated results appear:
Result
Molecular weight
Molar extinction
coefficient
Concentration factor Factor, based on the extinction coefficient, used to calculate the
Number of bases
% GC
To calculate the melting point of a DNA sequence
1. Enter the base sequence on the Oligo Calculator tab.
2. Enter appropriate values on the Melting tab:
Parameter Description
Oligo molarity Concentration of the Oligo in molar units.
Cation molarity Concentration of cations in the sample.
% Formamide Percentage concentration of formamide in the sample.
The calculated results appear:
Result Description
Salt-adjusted Calculated melting point of the base sequence, corrected for the
concentration of cation in the sample. This method does not
account for the effect of interaction between neighboring bases.
Nearest-neighbor Melting point of the base sequence when the effect of interaction
between neighboring bases is taken into account.
Contents
Description
Calculated base sequence molecular weight.
Calculated 260 nm extinction coefficient in ng-cm/microliter.
concentration of the base sequence.
Number of bases in the entered sequence.
Percentage of the total number of bases made up by guanine
and cytosine.
4
Life Science Applications
Nucleic Acid Labels
Evolution 200 Series User Guide 149