Thermo Scientific Evolution 200 Series User Manual page 467

Uv-vis instruments
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Thermo Scientific
Test
Wavelength Accuracy (Holmium
oxide liquid)
Wavelength Reproducibility
(Holmium oxide liquid)
Photometric Accuracy
(Customized)
Resolution (Toluene/Hexane)
Stray Light (KCl, 198 nm), Stray
Light (KI, 220 nm), Stray Light
(NaI, 220 nm), and Stray Light
(NaNO2, 340 nm)
Photometric Noise: 0A
(260 nm), Photometric Noise:
1A (260 nm), and Photometric
Noise: 2A (260 nm)
Baseline Flatness (Abs,
800-200 nm)
Photometric Drift
Description
Locates the peaks of a holmium oxide standard and
displays their measured and allowed range of
wavelengths. A holmium oxide solution prepared in
dilute perchloric acid has well defined absorption peaks
throughout the UV-visible range. These peaks are a
property of the material and serve as a wavelength
standard. The wavelengths and tolerance values are
cannot be changed.
Measures the ability of the spectrophotometer to return
to an identical wavelength in a repeatable manner using
a holmium oxide standard. A holmium oxide solution
prepared in dilute perchloric acid has well defined
absorption peaks throughout the UV-visible range.
These peaks are a property of the material and serve as a
wavelength standard. The wavelengths and tolerance
values are cannot be changed.
Automates the comparison of measured absorbance (or
%T) of photometric accuracy standards with their
certified values. Use Photometric Accuracy standards
calibrated to known absorbance values at specified
wavelengths. If a custom standard is being used instead
of a CVC, enter its wavelength, absorbance and
tolerance values in the boxes to the right.
Measures the ability of the spectrophotometer to resolve
adjacent features in a spectrum. Use a 0.02% (v/v)
solution of toluene in hexane and a hexane blank. The
wavelengths and tolerance values cannot be changed.
Compare measured stray light at the indicated
wavelength with the allowed range of values. Use the
Stray Light standard designed to measure stray light at
the indicated wavelength.
Measure the amount of noise at 260 nm. For
Photometric Noise: 0A (260 nm), use an empty cell
holder. For the latter two tests, use a 1A and 2A
metal-on-quartz filters suitable for the UV region for
these tests.
Measures the flatness of a baseline scan over the region
800 nm to 200 nm with smoothing applied to the data.
Use an empty cell holder.
Measures the absorbance drift of the instrument over a
one-hour period. Use an empty cell holder and let the
instrument warm up fully before initiating this test.
32
Performance Verification
PV Test Descriptions
Evolution 200 Series User Guide
453

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