Unique Screen Features; Making Protein A280 Measurements - Thermo Scientific Evolution 200 Series User Manual

Unique Screen Features

Making Protein A280 Measurements

Thermo Scientific
Accessories Tab for Protein A280
Feature
Type
ε/1000 and Molecular
Weight (kDa)
Ext. Coeff, ε 1% (L/g-cm)
Conc.
A280
260/280
Related Topics
Overview
Application Settings
Making Protein A280 Measurements
While performing this procedure,
spectral display.
To make a Protein A280 measurement
1. Click Measure Protein A280 in
Note To start an analysis immediately after viewing or changing the template settings,
click the Measure action button instead and skip to step 3.
2. Click Measure.
The right pane in Measure Protein A280 may include:
Description
Type of analysis to perform.
ε/1000 and molecular weight for the Other protein (ε &
MW) sample type.
Extinction coefficient and ε 1% for the Other protein (ε
1%) sample type.
Concentration based on absorbance at 280 nm, after
correction is applied, and the selected extinction coefficient.
Absorbance at 280 nm for the protein sample being
measured.
Ratio of absorbance at 260 nm and 280 nm.
click here for information about features to the right of the
Protein A280.
4
Life Science Applications
Protein A280
Evolution 200 Series User Guide 167