Frequently Asked Questions - Thermo Scientific Evolution 200 Series User Manual

Frequently Asked Questions

Thermo Scientific
Related Topics
Using the nanoCell
Cleaning the nanoCell
Storing the nanoCell
Frequently Asked Questions
Frequently Asked Questions
Q1 My nanoCell has two numbers on the lid (Factor 50 and LP 0.2 mm). What do these
numbers mean?
A1 Factor 50
This is the correction factor for this lid. If a 1 cm cuvette is used and equations are set
up for a 1 cm pathlength, multiply the 1.0 mm pathlength result by this factor (1 cm
result x 50, in this case) to get the correct concentration result. This is a factor of 50
reduction, or 50-fold dilution. See
LP 0.2 mm (Light Path 0.2 mm)
This is the pathlength of the nanoCell or the total distance the light travels through
the accessory. This distance is necessary for concentration calculations using the
Beer-Lambert law, which relates the absorbance of the solution to its concentration.
See Introduction to Small Volume Measurements
Q2 When using the nanoCell, is the correction for the pathlength (0.2 mm or 1.0 mm)
made automatically by the spectrophotometer, or does the end-user have to make this
correction?
A2 No. There is no mechanism for the instrument to know what pathlength nanoCell is
installed. The user will need to correct for the pathlength using the factor.
Q3 If the end-user has to make the correction, what is the correction for each nanoCell
pathlength?
A3 See Pathlength Correction Factors
most life science assays.
Q4 Does the quartz surface at the top of the nanoCell accessory and in the lid have to be
cleaned of all chemicals and fingerprint residue?
A4 Yes. The quartz sample loading area of the nanoCell accessory and the mirror in the
lid are the same as a traditional quartz cuvette. See
Pathlength Correction Factors.
and Pathlength Correction
for a comprehensive list of correction factors for
Cleaning the
Evolution 200 Series User Guide
18
nanoCell
Factors.
nanoCell.
289