Thermo Scientific Evolution 200 Series User Manual page 176

4
Life Science Applications
Protein A280
Measurement Tab for Protein A280
162 Evolution 200 Series User Guide
Feature
Protein A280 Type
Pathlength
Related Topics
Measurement Tab for Protein A280
Instrument Tab for Protein A280
Accessories Tab for Protein A280
Samples Tab for Protein A280
Description
Type of analysis to perform.
1 Abs = 1 mg / mL is a general reference setting based on a 0.1%
(1 mg/ml) protein solution. It produces an absorbance of 1.0 A at
280 nm (where the pathlength is 10 mm, or 1 cm).
BSA is a Bovine Serum Albumin reference. Protein concentrations
are calculated using the mass extinction coefficient of 6.7 at
280 nm for a 1% (10 mg/ml) BSA solution.
IgG is an IgG reference. Protein concentrations are calculated using
the mass extinction coefficient of 13.7 at 280 nm for a 1%
(10 mg/ml) IgG solution.
For Lysozyme, protein concentrations are calculated using the mass
extinction coefficient of 26.4 at 280 nm for a 1% (10 mg/ml)
Lysozyme solution.
Other protein (ε & MW) is the entered molar extinction
-1 -1
coefficient (M cm ) and molecular weight (MW) in kilodaltons
(kDa) for the respective protein reference. The maximum values are
99999000 for ε and 9999000 for MW.
Other protein (ε 1%) is the mass extinction coefficient (l g
for a 10 mg/ml (1%) solution of the respective reference protein.
Enter an ε/1000 value.
Distance the light travels through the sample.
Click Settings in
Measurement tab in the right pane.
Protein A280 to display the
-1 -1
cm )
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