Application Settings; Type Tab For Protein A280; Measurement Tab For Protein A280; Instrument Tab For Protein A280 - Thermo Scientific Evolution 200 Series User Manual

Application Settings

Type Tab for Protein A280

Thermo Scientific
Tyrosine, tryptophan, phenylalanine and to a small extent lysine and Cys-Cys disulphide
bonds have an absorbance peak around 280 nm. This method does not require generation of
a standard curve and is ready for protein sample quantitation at software startup. Colorimetric
assays such as Pierce BCA, Pierce 660 nm, Bradford and Lowry are more commonly used for
uncharacterized protein solutions and cell lysates.
The Protein A280 application displays the UV spectrum, measures the protein's absorbance at
280 nm (A280), and calculates the concentration (mg/ml).
Related Topics
Application Settings
Unique Screen Features
Making Protein A280 Measurements
Type Tab for Protein A280

Measurement Tab for Protein A280

Instrument Tab for Protein A280

Accessories Tab for Protein A280

Samples Tab for Protein A280

Related Topics
Overview
Unique Screen Features
Making Protein A280 Measurements
Feature
Name
Description (optional) Description of the template.
To set data acquisition parameters for a
workbook, click Settings to access:
Click Settings in
the right pane.
Description
Application name.
4
Life Science Applications
Protein A280
Protein A280 to display the Type tab in
Evolution 200 Series User Guide
Protein A280
161