Using The Nanocell; Installing The Nanocell; Loading A Sample - Thermo Scientific Evolution 200 Series User Manual

Using the nanoCell

Installing the nanoCell

Thermo Scientific
The linear relationship between absorbance, pathlength, and concentration in the Beer
Lambert law means that if we decrease the pathlength by a factor of 10, the observed
absorption will also decrease by a factor of 10.
Note Reducing the pathlength from 1 cm to 1 mm is equivalent to a 10-fold dilution.
Note The factor(s) multiplied by absorbance measurements to give concentration values
need to be adjusted for a change in the pathlength. For common DNA, RNA, and
oligonucleotide concentration factors, see
Related Topics
Using the nanoCell
Cleaning the nanoCell
Pathlength Correction Factors
Frequently Asked Questions
The nanoCell accessory has two basic components: the nanoCell and the mirrored lid used for
the measurement. The nanoCell contains the optics and sample loading window and fits in a
standard 1 cm cell holder.
Any UV-Visible measurement can be made using the nanoCell over a wavelength range of
190-1100 nm. This includes scanning, fixed wavelength, and kinetics measurements. The
measurement range, reproducibility, and wavelength range of the nanoCell is determined by
the spectrophotometer used to make the measurement.
Contents
• Installing the nanoCell
•

Loading a sample

• Measurements
The nanoCell ships configured for a z-height (distance from the bottom of the cell to the
center of the light beam) of 8.5 mm. An adapter is included for 15 mm z-height instruments.
The adapter simply screws on the base of the nanoCell accessory.
Pathlength Correction Factors.
Evolution 200 Series User Guide
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